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A. in B. on C. at D. without

The correct preposition to fill in the blank is “without”, which means “in the absence of” or “not accompanied by”. The complete sentence would be “It cannot be done without offence”, which means that it is impossible to do something without causing offense or upset to someone. The other prepositions given as options — “in”, “on”, and “at” — do not fit in the context of the sentence and would not convey the intended meaning.

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